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a OVCAR8 cells were being incubated with ten μM EdU, just before getting treated with car, 100 nM CX-5461, a hundred nM BMN-673 or The mixture of each for 24 h. Co-IF for γH2AX and RAD51 was executed. Cells ended up incubated for 30 minutes at space temperature with Simply click-IT response, washed with PBS then counterstained with DAPI